Thiamine improves in vitro propagation of sweetpotato [Ipomoea batatas (L.) Lam.] – confirmed with a wide range of genotypes

Abstract In vitro propagation of Plant Genetic Resources is a basic step for routine genebank and biotechnology research activities. Accelerating growth rooting in plants contributes to an improvement process efficiency plant quality. the present study effect supplemental thiamine explant size on biometric variables, ion content sap, chlorophyll leaves moisture were assessed replicated trial group seven sweetpotato accessions validated set other 45 accessions. It was shown that adding 0.1 mg L −1 modified Murashige Skoog culture medium significantly increased height, root length, number nodes shoot plants. No significant differences observed N0 3 − , K + Na Ca ++ —ion nor leaf area, chlorophyll, or between grown with without thiamine. Uninodal stem segments showed thiamine-free lower growth, reduced nodes, than from uni- binodal thiamine-supplemented medium. A subsequent experiment tested all parameters above non-replicated screen diverse With this germplasm, average length by 41 51%, respectively compared control treatment, confirming generally beneficial culture.